Gene description for FLOT1
Gene name	flotillin 1
Gene symbol	FLOT1
Other names/aliases	-
Species	Homo sapiens
Experiment description of studies that identified FLOT1 in Breast milk
1 Experiment ID 574 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting Mass spectrometry [LTQ-Orbitrap Elite, Q-Exactive] PubMed ID 27601599    Organism Homo sapiens Experiment description Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components Authors Van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH Journal name MCP Publication year 2016 Sample Breast milk Sample name Breast milk - normal Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.12-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV160000: EV-METRIC:50%
2 Experiment ID 950 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 27601599    Organism Homo sapiens Experiment description Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. Authors van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH Journal name Mol Cell Proteomics Publication year 2016 Sample Breast milk Sample name Milk - derived EVs - D1 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.12-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV160000: EV-METRIC:50%
3 Experiment ID 951 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 27601599    Organism Homo sapiens Experiment description Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. Authors van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH Journal name Mol Cell Proteomics Publication year 2016 Sample Breast milk Sample name Milk - derived EVs - D2 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.12-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV160000: EV-METRIC:50%
4 Experiment ID 952 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 27601599    Organism Homo sapiens Experiment description Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. Authors van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH Journal name Mol Cell Proteomics Publication year 2016 Sample Breast milk Sample name Milk - derived EVs - D3 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.12-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV160000: EV-METRIC:50%
5 Experiment ID 957 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 27601599    Organism Homo sapiens Experiment description Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. Authors van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH Journal name Mol Cell Proteomics Publication year 2016 Sample Breast milk Sample name Milk - derived EVs - pooled Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.12-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV160000: EV-METRIC:50%
6 Experiment ID 953 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry Western blotting PubMed ID 27601599    Organism Homo sapiens Experiment description Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. Authors van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH Journal name Mol Cell Proteomics Publication year 2016 Sample Breast milk Sample name Milk - derived EVs - D4 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.12-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV160000: EV-METRIC:50%
7 Experiment ID 954 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry Western blotting PubMed ID 27601599    Organism Homo sapiens Experiment description Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. Authors van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH Journal name Mol Cell Proteomics Publication year 2016 Sample Breast milk Sample name Milk - derived EVs - D5 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.12-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV160000: EV-METRIC:50%
8 Experiment ID 955 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry Western blotting PubMed ID 27601599    Organism Homo sapiens Experiment description Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. Authors van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH Journal name Mol Cell Proteomics Publication year 2016 Sample Breast milk Sample name Milk - derived EVs - D6 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.12-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV160000: EV-METRIC:50%
9 Experiment ID 956 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry Western blotting PubMed ID 27601599    Organism Homo sapiens Experiment description Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. Authors van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH Journal name Mol Cell Proteomics Publication year 2016 Sample Breast milk Sample name Milk - derived EVs - D7 Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.12-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV160000: EV-METRIC:50%
10 Experiment ID 2198 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 33732416    Organism Homo sapiens Experiment description Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses Authors Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. Journal name J Extracell Vesicles Publication year 2021 Sample Breast milk Sample name Eluate 1 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
11 Experiment ID 2199 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 33732416    Organism Homo sapiens Experiment description Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses Authors Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. Journal name J Extracell Vesicles Publication year 2021 Sample Breast milk Sample name Eluate 2 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
12 Experiment ID 2200 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 33732416    Organism Homo sapiens Experiment description Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses Authors Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. Journal name J Extracell Vesicles Publication year 2021 Sample Breast milk Sample name Eluate 3 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
13 Experiment ID 2201 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 33732416    Organism Homo sapiens Experiment description Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses Authors Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. Journal name J Extracell Vesicles Publication year 2021 Sample Breast milk Sample name Eluate 4 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
14 Experiment ID 2202 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 33732416    Organism Homo sapiens Experiment description Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses Authors Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. Journal name J Extracell Vesicles Publication year 2021 Sample Breast milk Sample name Eluate 5 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
15 Experiment ID 2203 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 33732416    Organism Homo sapiens Experiment description Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses Authors Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. Journal name J Extracell Vesicles Publication year 2021 Sample Breast milk Sample name Eluate 6 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
16 Experiment ID 2204 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 33732416    Organism Homo sapiens Experiment description Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses Authors Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. Journal name J Extracell Vesicles Publication year 2021 Sample Breast milk Sample name Eluate 7 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
17 Experiment ID 2205 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 33732416    Organism Homo sapiens Experiment description Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses Authors Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. Journal name J Extracell Vesicles Publication year 2021 Sample Breast milk Sample name Eluate 8 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
18 Experiment ID 2206 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 33732416    Organism Homo sapiens Experiment description Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses Authors Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. Journal name J Extracell Vesicles Publication year 2021 Sample Breast milk Sample name Eluate 9 Isolation/purification methods Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -